Journal: bioRxiv

Article Title: Polycystin-1 C-Terminus Regulates Protein Synthesis-Related Pathways in Cardiomyocytes

doi: 10.64898/2026.03.21.713243

Figure Lengend Snippet: A . Maximum z-projected images from immunofluorescence confirm endogenous PC1 C-terminal localization with a striated pattern that resembles cardiomyocyte sarcomeres (white, 161F antibody) in hiPSC-CMs. siRNA-mediated PKD1 knockdown demonstrates antibody specificity. DAPI (blue) is used to label nuclei in all panels except panel D-E . Panels B–F are single confocal optical sections. The far-right panels show the raw fluorescence intensity profile for panel A and the normalized fluorescence intensity profiles for panels B, C , and F . B . Endogenous PC1 C-terminus (magenta) localizes in a striated pattern alternating with α-actinin staining (green), consistent with sarcomeric alignment. C . Endogenous PC1 C-terminus (magenta) shows minimal colocalization with the sarco-endoplasmic reticulum marker (KDEL, green). D, E . Representative live-cell images of hiPSC-CMs and adult mouse ventricular CMs expressing viral constructs encoding mCherry or mCherry-PC1-CT. PC1-CT localizes with a striated pattern and, in some cells, nuclear localization (zoom panels were renormalized to highlight localization). F . Localization of mCh-PC1-CT in adult vCMs aligns with Z-line proteins α-actinin (upper panel) and desmin (lower panel).

Article Snippet: Primary antibodies mouse anti-human PC1-CT (161F, 1:200) [ ], rabbit anti-α-actinin-2 (1:200, Invitrogen, #701914), rabbit anti-mCherry antibody (1:200, Cell Signaling, #43590), mouse anti-α-actinin2 (1:200, Invitrogen, #MAI-22863), mouse anti-desmin (1:100, Invitrogen, #MAS-13259), and rabbit anti-KDEL (1:100, Invitrogen, #PA1-013).

Techniques: Immunofluorescence, Knockdown, Fluorescence, Staining, Marker, Expressing, Construct